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. 2022 Sep 18;27(18):6103. doi: 10.3390/molecules27186103

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Flow cytometric analysis of the HYAL4 protein on the surface of L6 cells. Representative line graphs of cellular staining are shown. Staining of L6 cells was performed using anti-HYAL4 antibody (clone A-7) (blue) or normal mouse IgG (red) under nonpermeabilized conditions. The binding of these antibodies to the epitopes on the cell surface was visualized by flow cytometry after incubating with Alexa Fluor 488-conjugated secondary antibody. Increased expression of HYAL4 on the cell surface was noted.