Table 1.
Yields of CS disaccharides in CHO cells overexpressed with mouse HYAL4.
| Disaccharide | Mock 1 | Hyal4 1 |
|---|---|---|
| ΔHexA-GalNAc 2 | ND 3 | ND |
| ΔHexA-GalNAc(4-O-sulfate) | 224.4 | 77.2 |
| ΔHexA-GalNAc(6-O-sulfate) | 2.3 | 1.1 |
| ΔHexA(2-O-sulfate)-GalNAc(6-O-sulfate) | ND | ND |
| ΔHexA-GalNAc(4,6-O-disulfate) | ND | ND |
| Total | 226.7 | 78.3 |
1 The values are given as pmol/mg protein and represent the mean of those obtained from two independent experiments. 2 CS is composed of repeating disaccharide units of GlcA and GalNAc with sulfations at various positions. Digestion of CS with bacterial CS-lyase into disaccharide units converts GlcA into unsaturated hexuronic acid (ΔHexA). 3 ND, not detected.