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Asymmetric localization of FtsZ in a dnaA46 mutant. WM1270 cells shifted to 42°C for 3 h in LB plus thymine were fixed and immunostained for FtsZ. (A) FtsZ staining in green; (B) nucleoids stained with DAPI, pseudocolored red; (C) digital overlay of panels A and B; (D) overlay of panel A and a phase contrast image. Bar, 5 μm.
