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. 2022 Sep 9;13:953151. doi: 10.3389/fimmu.2022.953151

Figure 2.

Figure 2

CD4+ iNKT cells with a mixed type 1 and type 2 signature are preferentially induced during acute infection. Hepatic iNKT cell subsets (n=4-8 mice per time point) were analyzed by flow cytometry at different stages of NrHV infection. (A) FACS plots showing T-bet and GATA-3 expression in CD4+ and CD4- subsets at day 7 pi. (B) Total numbers of GATA-3+Tbet+ and GATA-3-Tbet+/- CD4+ (left graph) and CD4- (right graph) iNKT cell subsets in the liver at multiple stages of infection and controls. (C) CD69 expression on GATA-3+Tbet+ and GATA-3-Tbet+/- subsets of CD4+ (upper graphs) and CD4- (lower graphs) iNKT cells. (D) FACS plots showing CD69 expression levels on iNKT cell subsets. (E) Ki67+ expression in GATA-3+Tbet+ and GATA-3-Tbet+/- subsets of CD4+ (blue) and CD4- (orange) iNKT cells. Unpaired t test and one-way ANOVA: *p< 0.5, **p<0.01, ***p<0.001.