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. 2022 Sep 9;13:953151. doi: 10.3389/fimmu.2022.953151

Figure 3.

Figure 3

iNKT cell subsets produce type 2 cytokines during acute NrHV infection. Hepatic leukocytes were isolated from C57BL/6 mice (controls, day 3, 7, 14 pi; n=4-8 mice per time point) and immediately analyzed (no stimulation) for intracellular cytokines, granzyme B and perforin by flow cytometry. (A) Histograms of IL-13, IL-4 and IFN-γ expression by iNKT cell subsets at day 3 pi compared to controls. (B, C) Frequencies of hepatic CD4+ (B) and CD4- (C) iNKT cells producing IL-13, IL-4 or IFN-γ within the respective cell subset are shown. (D) Kinetics of IFN-γ, IL-13 and IL-4 serum levels during acute NrHV infection (n=10 mice) were determined by cytometric bead array. (E) Representative FACS plots of perforin and granzyme B expression. (F) Hepatic iNKT cell subsets were analyzed for the co-expression of cytokines with T-bet and GATA-3 at day 7 pi. T cells from the same time point are shown as comparison. Unpaired t test and one-way ANOVA: *p< 0.5, **p<0.01, ***p<0.001.