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. 2022 Sep 9;13:953151. doi: 10.3389/fimmu.2022.953151

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Copyright © 2022 Raus, Lopez-Scarim, Luthy and Billerbeck

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hepatic lymphocyte subsets in CD1d^-/- mice during acute infection. A flow cytometric analysis of hepatic lymphocytes in WT and CD1d^-/- mice (n=4-8 per time point) was performed at day 7 and 14 pi and in uninfected controls. (A) Frequencies of B cells, NK cells, CD4+ and CD8+ T cells. (B) Total numbers of hepatic CD4+ and CD8+ T cells at day 7pi. (C) CD69 and T-bet expression of CD4+ and CD8+ T cells. (D) Granzyme B and perforin (upper graphs) and IFN-γ (lower graphs) expression of T cell subsets after unspecific stimulation with PMA/Ionomycin. (E) Representative FACS plots of granzyme B/perforin and IFN-γ expression in CD8+ T cells at day 14 pi. Unpaired t test: *p< 0.5, **p<0.01.