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. 2022 Jul 25;71(10):2197–2208. doi: 10.2337/db22-0233

Figure 5.

Figure 5

Altered molecular expression is responsible for the diminished BSM contraction force in SMIR+/− and SMIR−/− mice bladder. A: Western blot of Chrm3, P2x1, Itgβ1, Sm22, and Cav1.2 proteins from wild-type (WT), SMIR+/−, and SMIR−/− mice bladders (n = 4). β-Actin is used as loading control for normalization and quantitated data are shown as B–F. Data are shown as boxes and whiskers, the centerline is the median of the data set, the box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed with use of Student t test. *P < 0.05 and **P < 0.01.