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. 2022 Jul 25;71(10):2197–2208. doi: 10.2337/db22-0233

Figure 6.

Figure 6

Insulin signaling abnormalities in SMIR+/− and SMIR−/− mice bladders. A: Western blot of Insr, Akt, p-Akt, FoxO1, FoxO3, Fox6, mTOR, and p-mTOR proteins from wild-type (WT), SMIR+/−, and SMIR−/− mice bladders (n = 4). β-Actin is used as loading control for normalization, and quantitated data are shown in B–I. Data are shown as boxes and whiskers. The centerline is the median of the data set, the box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed with use of Student t test. *P < 0.05 and **P < 0.01.