TABLE 1.

Strains used in this work^a

Strain	Genotype	Reference or sourceb
FGSC26	biA1 veA1	FGSC
RMS011	pabaA1 yA2 ΔargB::trpCΔB veA1 trpC801	36
CLK20	biA1 ΔcatA::argBΔA metG1 ΔcatB::argBΔB veA1	Progeny from cross TRN1 × CLK15 (this work) (FGSC strain A1055 and ATCC MYA-116)
TLK61	pabaA1 yA2 ΔcatC::argBΔC ΔargB::trpCΔB trpC801 veA1	Obtained by transforming strain RMS011 with linear pLK20 (this work)
TLK12	pabaA1 yA2 ΔargB::trpCΔB ΔcatB::argBΔB trpC801 veA1	23 (FGSC strain A1054 and ATCC MYA-118)
CLK35	pabaA1 yA2 biA1 ΔcatC::argBΔC ΔcatA::argBΔA ΔcatB::argBΔB veA1	Progeny from cross CLK20 × TLK61 (this work)
CLK14	biA1 ΔcatA::argBΔA metG1 ΔcatB::argBΔB veA1	Progeny from cross CLK12 × TLK12 (this work)
CLK15	biA1 metG1 ΔcatB::argBΔB veA1	Progeny from cross CLK12 × TLK12 (this work)
RYC17	ΔargB::trpCΔB ΔcatA::argB veA1	Partial genotype 7
RYC16	ΔargB::trpCΔB ΔcatA::argB ΔcatB::argB veA1	Partial genotype 7
CLK36	pabaA1 biA1 ΔcatC::argBΔC ΔcatA::argBΔA metG1 ΔcatB::argBΔB veA1	Progeny from cross CLK20 × TLK61 (this work)

^aTo obtain triple catA catB catC mutants, strains CLK20 and TLK61 were crossed. Master plates containing progeny from this cross were screened for the lack of CatB, using 20 mM H₂O₂, as described previously (23). Of 94 strains, 37 lacked catB and used to extract genomic DNA. DNA samples were screened for catC disruption by PCR, using oligonucleotides catC10 (5′AAGATTGGGTCGAAGCGG3′) and argB1 (5′CATAAGTCCGCCAGCAGG3′). The lack of CatA and CatB activities was confirmed by Zymogram analysis. 

^bFGSC, Fungal Genetics Stock Center.