Figure 4.

Technical research route of STS in basidiomycetes. The STS genome mining of basidiomycetes can be divided into three steps. The first step of genome mining is mainly based on the whole gene sequence, using bioinformatics tools to mine and predict the biosynthetic gene cluster. The second step is to obtain the target gene from the biosynthetic gene cluster of STS, and then heterologously express the target gene to obtain the product. The final step is to purify and identify the product to determine whether the gene is an STS.