Figure 1.
Basal GUS activity in leaves and roots of 3-week-old T3 transgenic soybean containing the individual promoter-GUS construct under unstressed condition. (A) Histochemical staining for GUS activity in transgenic soybean and wild-type (WT) plants. (B) Fluorometric assay for GUS activity in transgenic soybean plants. (C) Quantitative real-time RT-PCR (qRT-PCR) analysis for GUS expression in transgenic soybean plants. The relative levels of transcripts were normalized to soybean ubiquitin gene (GmUBI3). Three independent transgenic lines (L1, L2, and L3) were used for 4 × M1.1 and 4 × M2.3 promoter-GUS constructs. One transgenic line (L1) was used for 35S and minimal (Min) 35S promoter-GUS constructs. Bars represent mean values of six biological replicates ± standard error.