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Primer-templated oligomer synthesis. (a) In PCR, a primer anneals with the template to provide a reactive chain end for attachment of the complementary monomer by a polymerase enzyme (pol). (b) In a synthetic system, a covalent base-pair can be used to load the primer, providing a reactive chain end bound to the template. Noncovalent base-pairing can then then be used to select the complementary monomer for chain elongation.
