Table 4. Proteins Used in This Study with Respective PDB ID and Number of Available Amine Reactive Sites.
| Protein | PDB ID | Number of amine reactive sites |
|---|---|---|
| Lysozyme | 1LYZ | 6–7 |
| Chymotrypsin | 4CHA | 15 |
| Butylcholinesterase (BChE) | 6I2T | 136 |
| Bovine Serum Albumin (BSA) | 4F5S | 60 |
| Diisopropyl Fluorophosphatase (DFPase) | 1PJX | 25 |
| Horseradish Peroxidase (HRP) | 1H57 | 7 |
| Methioninase (METase) | 1UKJ | 33 |
| Human Arginase | 1WVA | 23 |
| Human Growth Hormone | 3HHR | 10 |
| Α-lactalbumin | 1A4 V | 12 |
| Single Chain Variable Fragment (scFv) | 6PIL | 10 |
| Tetrameric phenylalanine ammonia lyase (rAV-PAL). | 5LTM | 16a |
| Interferon-a 2a | 1ITF | 8a |
Original proteins possess 19 reactive sites (rAV-PAL) and 10 reactive sites (interferon-a 2a), respectively. Calculated reactive sites were reduced to 16 and 8, respectively, due to missing residues in the PDB structure.