Figure 2.

BdAtg1 is indispensable for autophagy. (A) GFP-BdAtg8 localization in the mycelia of the wild-type strain LW03 and BdATG1 deletion mutant ΔBdAtg1 under the conditions of sufficient or lack of nitrogen source. (B) GFP-BdAtg8 proteolysis of each strain (starvation for 0 and 8 h) was detected by Western blotting using anti-GFP antibody. GAPDH was used as an internal reference. (C) The percentage of GFP on the total of GFP and GFP-BdAtg8. Error bars indicate standard deviation from three independent experiments. Values on the bars followed by the same letter are not significantly different at p = 0.05.