Skip to main content
. 2022 Sep 9;15:942548. doi: 10.3389/fnmol.2022.942548

FIGURE 2.

FIGURE 2

Calcium induced Ca2+ release genes IP3 and ryanodine receptors are required for cold-evoked behaviors. (A,B) Transcriptomic analysis using microarray datasets. (A) Quantile normalized expression of RyR and IP3R in CIII md neurons. (B) Comparative analysis of IP3R and RyR expression between CIII md neurons and CIV md neuron or whole larva (WL). (C,D) Cold plate analysis of RyR and IP3R. (C) Drosophila larvae heterozygous mutants for either IP3R (ug3, ka901, and 90B0 alleles) or RyR (16, Q3878X, and Y4452X alleles). All statistical comparisons were made to w1118. NAverage = 49. We report %CT (left), CT duration in seconds (middle), CT magnitude (right). (D) IP3R or RyR knockdown specifically in CIII md neurons using 19–12GAL4 driver. Control conditions include: w1118, 19–12GAL4/+, and 19–12 > LucRNAi. We report %CT (left), CT duration in seconds (middle), CT magnitude (right). There is no statistical difference between the controls. Knockdown of RyR and IP3R was tested using multiple independent RNAi lines and comparisons made to 19–12GAL4/+. NAverage = 55. Significant differences indicated via asterisks, where *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.