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. 2022 Sep 16;14(9):2054. doi: 10.3390/v14092054

Figure 3.

Figure 3

Expression of cell type-specific and viral markers in VIN cells. (A) Expression of cell type-specific markers in VIN cells under 2D cultures. VIN cells were seeded in 48-well plates and collected after 24 h, then fixed in 4% paraformaldehyde and detected by immunofluorescence staining with primary antibodies against CK14, CK18, p63, Ki67, p53, and HPV18 E7. Scale bar, 100 μm. (B) Expression of cell type-specific and viral markers in VIN, HeLa and NVEC under 2D cultures. Cells were fixed and detected by DAB staining with primary antibodies against CK14 and HPV18 E7. The results of H&E and DAB staining were photographed under a microscope. Scale bar, 100 μm. (C) Expression of cell type-specific and viral markers under 3D matrigel cultures. Single-cell suspensions of VIN cells and HeLa cells were cultured in medium containing 2% matrigel for 10 days. The 3D cultures were fixed with 4% (w/v) paraformaldehyde, paraffin-embedded, sectioned using standard histological procedures, and detected by DAB staining with the primary antibodies against CK14, p63, Ki67, HPV18 E7, p53, involucrin, and filaggrin. The results of H&E and DAB staining were photographed under a microscope. Scale bar, 100 μm.