Figure 5.

Inhibitory Effect of Betulin on Inflammatory Mediators by Regulating 11β-HSD1 and C/EBP Activities. The HPDL cells (5 × 10³ cells/mL) were pretreated with or without the indicated concentration of betulin, after treatment with PG-LPS, it was cultured for 14 days. The result of mineralization was measured by alizarin red s (ARS) staining (A). The level of osteogenic induction specific genes (alp, opn, runx2) and proteins were analyzed by real-time PCR (B) and Western blot (C). The results were normalized to gapdh and β-actin expression. * p < 0.05 was considered significant differences between only PG-LPS treated groups.