FIG. 1.

SDS-PAGE and Western blotting analysis of cells expressing EvgA and protein X. Membrane and cytoplasmic fractions of E. coli KAM3 and KAM3/pQE30evgA were prepared. The membrane (P) and cytoplasmic (S) fractions were then subjected to SDS-PAGE on 15% polyacrylamide gels. After electrophoresis, the gels were stained with Coomassie brilliant blue (A). Then proteins were electroblotted onto poly-vinylidene difluoride membranes. Histidine-tagged EvgA was detected with antipolyhistidine antibodies (B).