Table 6.

Percent inhibition of two doses of the EOs on biofilm formation of A. baumannii, E. coli, L. monocytogenes, P. aeruginosa and S. aureus, at 0 and 24 h.

Time 0	A. baumannii	E. coli	L. monocytogenes	P. aeruginosa	S. aureus
E. occidentalis 10 μL/mL	83.92 ± 2,17 ****	20.54 ± 0.07 ****	0 ± 0	0 ± 0	2.02 ± 0.74
E. occidentalis 20 μL/mL	86.73 ± 1.16 ****	46.93 ± 0.87 ****	79.48 ± 0.36 ****	13.79 ± 1.00 ****	31.08 ± 0.73 ****
E. striaticalyx 10 μL/mL	35.57 ± 6.98 ****	0 ± 0	8.23 ± 0.69 ****	0.84 ± 0.20	48.25 ± 2.12 ****
E. striaticalyx 20 μL/mL	66.66 ± 0.94 ****	10.36 ± 4.61 ****	27.16 ± 4.11 ****	30.67 ± 2.49 ****	60.46 ± 0.66 ****
E. stricklandii 10 μL/mL	5.24 ± 0.65 **	1.12 ± 0.40	0 ± 0	0 ± 0	45.20 ± 1.92 ****
E. stricklandii 20 μL/mL	46.49 ± 3.62 ****	1.48 ± 0.27	36.86 0.78 ****	42.66 ± 1.33 ****	45.16 ± 2.01 ****
24 h	A. baumannii	E. coli	L. monocytogenes	P. aeruginosa	S. aureus
E. occidentalis 10 μL/mL	9.71 ± 0.67 ****	28.60 ± 1.04 ****	0 ± 0	47.97 ± 0.58 ****	49.93 ± 0.82 ****
E. occidentalis 20 μL/mL	32.45 ± 0.62 ****	40.35 ± 0.87 ****	67.75 ± 0.46 ****	56.20 ± 0.57 ****	52.42 ± 0.85 ****
E. striaticalyx 10 μL/mL	39.11 ± 0.38 ****	6.35 ± 0.73 ****	26.38 ± 1.35 ****	8.18 ± 0.57 ****	19.40 ± 0.79 ****
E. striaticalyx 20 μL/mL	45.23 ± 0.91 ****	31.21 ± 1.21 ****	34.14 ± 0.62 ****	17.43 ± 0.58 ****	23.29 ± 1.15 ****
E. stricklandii 10 μL/mL	25.72 ± 0.83 ****	0 ± 0	0 ± 0	9.23 ± 1.37 ****	29.10 ± 0.89 ****
E. stricklandii 20 μL/mL	58.83 ± 0.38 ****	29.22 ± 0.81 ****	42.28 ± 0.62 ****	51.60 ± 0.37 ****	36.04 ± 0.89 ****

The percentage of inhibition was calculated using the formula: OD_C-OD_S/ODC*100, where OD_C is the OD of the untreated bacteria, and ODs is the OD of the bacteria treated with samples. Results are reported as the mean ± SD of three experiments. *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.00001 vs. control (inhibition = 0), according to two-way ANOVA followed by Dunnet’s multiple comparison test, at the significance level of p < 0.05.