Table 7.

Percent inhibition of two doses of the EOs on biofilm metabolic activity of A. baumannii, E. coli, L. monocytogenes, P. aeruginosa and S. aureus, at 0 and 24 h.

Time 0	A. baumannii	E. coli	L. monocytogenes	P. aeruginosa	S. aureus
E. occidentalis 10 μL/mL	41.05 ± 3.27 ****	0 ± 0	0 ± 0	0 ± 0	57.44 ± 4.98 ****
E. occidentalis 20 μL/mL	55.18 ± 1.30 ****	7.70 ± 0.80 ****	44.69 ± 0.40 ****	14.33 ± 0.20 ****	72.03 ± 2.30 ****
E. striaticalyx 10 μL/mL	0 ± 0	12.50 ± 0.57 ****	96.01 ± 1.11 ****	44.35 ± 0.85 ****	67.18 ± 4.25 ****
E. striaticalyx 20 μL/mL	27.19 ± 1.19 ****	27.11 ± 1.32 ****	96.81 ± 1.31 ****	39.38 ± 0.85 ****	76.36 ± 0.99 ****
E. stricklandii 10 μL/mL	0.00 ± 0.00	0.00 ± 0.00	20.90 ± 0.67 ****	0 ± 0	79.44 ± 0.78 ****
E. stricklandii 20 μL/mL	42.06 ± 1.30 ****	21.28 ± 1.87 ****	97.67 ± 1.81 ****	60.65 ± 1.87 ****	83.02 ± 1.04 ****
24 h	A. baumannii	E. coli	L. monocytogenes	P. aeruginosa	S. aureus
E. occidentalis 10 μL/mL	0 ± 0	10.32 ± 0.57 ****	0 ± 0	0 ± 0	18.43 ± 0.24 ****
E. occidentalis 20 μL/mL	88.39 ± 1.50 ****	27.08 ± 0.68 ****	0 ± 0	9.73 ± 0.47 ****	30.54 ± 0.63 ****
E. striaticalyx 10 μL/mL	62.22 ± 1.26 ****	23.15 ± 0.76 ****	26.28 0.15 ****	44.40 ± 0.69 ****	0 ± 0
E. striaticalyx 20 μL/mL	67.91 ± 0.72 ****	24.05 ± 0.92 ****	26.55 ± 2.44 ****	45.11 ± 0.84 ****	0.68 ± 0.02
E. stricklandii 10 μL/mL	6.26 ± 0.22 ****	0 ± 0	27.15 ± 0.80 ****	42.53 ± 0.35 ****	0 ± 0
E. stricklandii 20 μL/mL	67.14 ± 0.15 ****	13.29 ± 0.34 ****	29.77 ± 0.53 ****	45.31 ± 0.88 ****	0 ± 0

The percentage of inhibition was calculated using the formula: OD_C-OD_S/ODC*100, where OD_C is the OD of the untreated bacteria, and ODs is the OD of the bacteria treated with samples. Results are reported as the mean ± SD of three experiments. *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.00001 vs. control (inhibition = 0), according to two-way ANOVA followed by Dunnet’s multiple comparison test, at the significance level of p < 0.05.