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HPLC chromatogram of a neutral lipid fraction from T. acidophilum HO-62. A Shiseido (Tokyo, Japan) Capcell pak silica UG80 column (4.6 by 250 mm) attached to an HPLC system (monitored at 248 nm) was used. The sample was separated using hexane-diethylether-trifluoroacetic acid (99:1:0.002, vol/vol/vol) at a flow rate of 0.86 ml · min−1.
