Figure 5.

Saq B₁ induces apoptosis in CRC cells. (A) DAPI staining was used to study the effect of Saq B₁ on apoptosis in SW480 cells. The arrows show nuclei that are condensed and fragmented within the cell. Scale bars = 20 µm. (B) Changes in MMP were detected using the JC-1 probe after SW480 cells were treated with different concentrations of Saq B₁. Representative images are shown. Scale bars = 100 µm. The fluorescence intensity of JC-1 was analyzed using Image J software, and the statistical graph was drawn. (C) Apoptosis in SW480 and SW620 cells treated with and without Saq B₁ was determined by flow cytometry using Annexin V-FITC/PI staining. (D) Western blot was used to analyze the expression levels of Bax and Bcl-2. * p < 0.05, ** p < 0.01, *** p < 0.001.