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Correlations between frequencies of FoxP3−Helios− and immune checkpoints in CD4+ T cells in CRC patients based on disease stages. Correlations between frequencies of CD4+FoxP3−Helios−Tregs with PD-1+ (A), TIM-3+ (B), LAG-3+ (C), and CTLA-4+ (D) in PBMCs, TILs, and NILs in CRC patients with early and advanced stages.
