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Concentration of sigI/ykoZ mRNA in B. subtilis 168 and BFA 251 grown in LB medium and SMM. A slot blot analysis of total RNA, which was isolated before (0 min) and 3, 6, and 9 min after a heat shock from 37 to 50°C (or 52°C, as indicated) is shown. The amount of total RNA used was 5, 2.5, or 1.3 μg as indicated.
