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2-D PAGE analysis of proteins present in the wild-type B. subtilis 168 (A) and in BFA 251 (B) grown in LB medium for 30 min after the heat shock. The GsiB spot was identified by comparison with a B. subtilis master gel (1), and the GsiB spot-containing sections of the gels are presented. For generating the images, 80-μg portions of protein extracts were fractionated by 2-D PAGE and the gels were stained with silver (12).
