Table 3.
Effect of Rumex hastatus crude extract (Rh.Cr) and fractions: n-hexane (Rh.n-Hex), ethyl acetate (Rh.ETAC), aqueous (Rh.Aq), rutin and loperamide against castor-oil induced diarrhea in mice.
| Treatment (mg/kg) |
No of Wet Feces | Total No of Feces | Average Weight of Wet Feces (gm) | Average Weight of Total Feces (gm) | % Inhibition of Defecation | % WWFO | % WTFO |
|---|---|---|---|---|---|---|---|
| Saline (10 mL/kg) + Castor-oil (10 mL/kg) | 7.2 ± 0.3 | 8.4 ± 0.24 | 0.44 ± 0.05 | 0.5 ± 0.01 | 0 | 0 | 0 |
| Rh.Cr (50 mg/kg) + Castor-oil (10 mL/kg) | 4.2 ± 0.09 | 6 ± 0.24 | 0.26 ± 0.06 | 0.38 ± 0.05 | 41.6 ** | 59.09 | 76 |
| Rh.Cr (100 mg/kg) + Castor-oil (10 mL/kg) | 1.4 ± 0.17 | 4.2 ± 0.1 | 0.06 ± 0.04 | 0.25 ± 0.03 | 80.5 *** | 13.6 | 50 |
| Rh.Cr (300 mg/kg) + Castor-oil (10 mL/kg) | 0 ± 0.0 | 2.5 ± 0.05 | 0 ± 0.0 | 0.14 ± 0.04 | 100 *** | 0 | 28 |
| Rh.n-Hex (50 mg/kg) + Castor-oil (10 mL/kg) | 3.9 ± 0.09 | 6.8 ± 0.2 | 0.24 ± 0.18 | 0.39 ± 0.11 | 45.8 ** | 54.5 | 78 |
| Rh.n-Hex (100 mg/kg) + Castor-oil (10 mL/kg) | 2.9 ± 0.05 | 5.5 ± 0.3 | 0.16 ± 0.27 | 0.32 ± 0.13 | 59.7 ** | 36.36 | 64 |
| Rh.n-Hex (300 mg/kg) + Castor-oil (10 mL/kg) | 1.4 ± 0.18 | 4 ± 0.06 | 0.06 ± 0.09 | 0.23 ± 0.19 | 80.5 *** | 13.6 | 46 |
| Rh.ETAC (50 mg/kg) + Castor-oil (10 mL/kg) | 5.5 ± 0.5 | 6.2 ± 0.4 | 0.33 ± 0.1 | 0.41 ± 0.12 | 23.6 * | 75 | 82 |
| Rh.ETAC (100 mg/kg) + Castor-oil (10 mL/kg) | 4.5 ± 0.24 | 5.2 ± 0.2 | 0.29 ± 0.04 | 0.31 ± 0.03 | 37.5 ** | 62 | 67.2 |
| Rh.ETAC (300 mg/kg) + Castor-oil (10 mL/kg) | 2.7 ± 0.23 | 5.3 ± 0.32 | 0.13 ± 0.45 | 0.3 ± 0.04 | 62.5 *** | 29.5 | 60 |
| Rh.Aq (50 mg/kg) + Castor-oil (10 mL/kg) | 7.2 ± 0.08 | 8.2 ± 0.12 | 0.44 ± 0.01 | 0.49 ± 0.05 | 0 | 100 | 98 |
| Rh.Aq (100 mg/kg) + Castor-oil (10 mL/kg) | 6.8 ± 0.05 | 7.5 ± 0.1 | 0.41 ± 0.06 | 0.45 ± 0.05 | 5.55 | 93.1 | 90 |
| Rh.Aq (300 mg/kg) + Castor-oil (10 mL/kg) | 6.5 ± 0.22 | 7.2 ± 0.1 | 0.40 ± 0.03 | 0.44 ± 0.05 | 9.72 | 90.1 | 88 |
| Rutin (50 mg/kg) + Castor-oil (10 mL/kg) | 4.2 ± 0.2 | 7 ± 0.30 | 0.27 ± 0.08 | 0.39 ± 0.05 | 41.6 ** | 61.36 | 78 |
| Rutin (100 mg/kg) + Castor-oil (10 mL/kg) | 1.4 ± 0.24 | 4.5 ± 0.16 | 0.07 ± 0.04 | 0.26 ± 0.01 | 80.5 *** | 15.9 | 52 |
| Rutin (200 mg/kg) + Castor-oil (10 mL/kg) | 0 ± 0.0 | 2.7 ± 0.03 | 0 ± 0.0 | 0.15 ± 0.02 | 100 *** | 0 | 30 |
| Loperamide (2 mg/kg) + Castor-oil (10 mL/kg) | 0 ± 0.0 | 2.2 ± 0.2 | 0 ± 0.0 | 0.11 ± 0.03 | 100 *** | 0 | 22 |
Values expressed as mean ± SEM (n = 5). One-way ANOVA with post-hoc Tukey’s test. p < 0.001 vs. saline group, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. castor oil group.