TABLE 1.
Growth of C. ulcerans strains in the presence of hemin or hemoglobina
| Strain/plasmid (gene) |
A600 in the presence
of indicated iron source
|
||||
|---|---|---|---|---|---|
| None | Hemin
(μM)
|
Hemoglobin (μM)
|
|||
| 0.1 | 1.0 | 0.1 | 1.0 | ||
| CU712 (wt)/pCM2.6 (vector) | 0.1 | 1.8 | 6.4 | 4.4 | 6.1 |
| CU77/pCM2.6 | 0.2 | 0.2 | 4.8 | 0.6 | 4.9 |
| CU77/pCUT27 (hmuT) | 0.2 | 1.2 | 4.9 | 3.8 | 5.1 |
| CU77/pCUHM (hmuTUV) | 0.2 | 1.8 | 4.4 | 4.6 | 5.1 |
| CU77/pHMU1.26 (hmuUV) | 0.3 | 0.5 | 4.4 | 0.8 | 4.9 |
| CUR.2/pCM2.6 | 0.2 | 0.2 | 5.4 | 0.6 | 5.5 |
| CUR.2/pCUT27 (hmuT) | 0.2 | 0.2 | 5.3 | 0.8 | 5.4 |
| CUR.2/pCUHM (hmuTUV) | 0.3 | 1.3 | 5.4 | 3.3 | 5.9 |
| CUR.2/pHMU1.26 (hmuUV) | 0.2 | 0.2 | 5.1 | 0.4 | 5.2 |
| CU44/pCM2.6 | 0.1 | 0.1 | 2.8 | 0.2 | 3.0 |
| CU44/pCUHM (hmuTUV) | 0.1 | 1.6 | 4.0 | 2.7 | 3.9 |
| CU44/pHMU1.26 (hmuUV) | 0.1 | 1.4 | 3.6 | 2.2 | 4.2 |
a
Strains were initially grown overnight in HIBTW and then passaged 1/100 into HIBTW containing 200 μg of EDDA per ml and grown for 18 h. These cultures were then passaged 1/100 into HIBTW-EDDA medium containing hemin or hemoglobin or no iron source. Absorbance (A600) measurements were done on 18-h cultures, and the values shown are the averages of three independent experiments. Standard deviations did not vary by more than 20% from the mean.