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. 2022 Sep 23;20:426. doi: 10.1186/s12967-022-03633-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Overexpression of MIR99AHG suppresses EMT and fibrosis. A Analysis of LncRNA MIR99AHG expression in LUAD and normal lung tissues using the GEPIA database. B Analysis of the relationship between LncRNA MIR99AHG expression and survival of LUAD patients using the Kaplan–Meier plotter. C RT-qPCR detection of LncRNA MIR99AHG expression. D RT-qPCR to detect the stable overexpression efficiency of LncRNA MIR99AHG. E RT-qPCR to detect the expression of E-Cadherin, Vimentin, ACTA2 and COL1A1 after overexpression of LncRNA MIR99AHG. F, G The protein expression of TGF-β1, E-Cadherin, Vimentin, ACTA2 and COL1A1 was detected by WB and statistically quantified by ImageJ software. H, I After overexpression of LncRNA MIR99AHG, cell migration was monitored by wound scratch assay. Statistical data are shown. J, K After overexpression of LncRNA MIR99AHG, cell invasion was measured by invasion assay. Cell invasion was determined by invasion assay and counted by ImageJ counting. L, M Immunofluorescence assay was performed to detect the expression of fibrosis-related proteins after overexpression of LncRNA MIR99AHG. Statistical data are shown. N Subcutaneous tumorigenesis demonstration in nude mouse. O Tumor weights were represented as the means of tumor weights ± SD. P RT-qPCR to confirm the overexpression of LncRNA MIR99AHG in tumor tissues. Q, R WB to detect the expression of TGF-β1, E-Cadherin, Vimentin, ACTA2 and COL1A1 in MIR99AHG stabled overexpression tumor tissues. Statistical data are shown. S The tumor sections underwent IHC staining and H&E staining. *P < 0.05, **P < 0.01