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. 2022 Sep 23;20:426. doi: 10.1186/s12967-022-03633-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — USP4 is a direct target gene of miR-136-5p. A, B TargetScan and mirDIP prediction of the target gene of miR-136-5p. Funrich made a Wayne diagram. C WT and MUT of the 3’UTR of USP4 luciferase reporter plasmids are shown schematically. D Luciferase activity analysis in HEK293T cells after transfection according to experimental subgroups (n = 3). E Enrichment of USP4 in A549 cells transfected with miR-136-5p-WT and miR-136-5p-MUT plasmids. F Luciferase reporters containing USP4-Luc-WT were co-transfected with or without miR-136-5p, and USP4, Luciferase activity was determined using a dual luciferase reporter system. G, H After treatment of A549 with miR-136-5p inhibitor, the protein level of USP4 was detected by WB and quantitatively analyzed by ImageJ.