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. 2022 Sep 23;20:426. doi: 10.1186/s12967-022-03633-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Overexpression of ACE2 is involved in EMT and fibrosis in LUAD. A Kaplan–Meier plotter analysis of ACE2 expression in relation to survival in lung cancer patients. B Higher ACE2 levels were positively correlated with cancer stage. C RT-qPCR detection of ACE2 in BEAS-2B and A549. D, E WB detection of ACE2 protein expression in BEAS-2B and A549, and statistical quantification of ACE2 protein expression by ImageJ software. F After overexpression of ACE2, RT-qPCR detection of ACE2 mRNA expression levels. G, H After overexpression of ACE2, WB to detect ACE2 protein expression levels after overexpression of ACE2. ImageJ software for statistical quantification. I RT-qPCR to detect EMT and fibrosis marker expression after overexpression of ACE2. J, K WB to detect the protein expressions of TGF-β1, E-cadherin, vimentin, ACTA2 and COL1A1 after overexpression of ACE2. ImageJ software for statistical quantification of protein expression. L Functional enrichment of ACE2 analysis. M, N Cell migration was monitored by wound scratch assay after overexpression of ACE2. O, P Cell invasion was measured by invasion assay after overexpression of ACE2 and counted statistically by ImageJ. Q, R Immunofluorescence assay to detect fibrosis-associated protein expression after overexpression of ACE2. Statistical data are shown