Fig. 3.

EIF4A3 induced circ_0086296 expression in HUVECs. A–C The potent area of EIF4A3 binding with the flanking sequences of the UHRF2 mRNA transcript was predicted. D The interaction between EIF4A3 and the circ_0086296 upstream region was detected. E RIP assay to demonstrate EIF4A3 binding with the putative sequences. F The levels of circ_0086296 in HUVECs transfected with sh-NC, sh-EIF4A3, pcDNA-NC, or pcDNA-EIF4A3, **p < 0.001 versus the OE-NC group. ^##p < 0.001 versus the shNC group; and G the levels of EIF4A3 in HUVECs infected with circ_0086296 overexpression vector or sh-circ_0086296 vectors were determined via qRT-PCR. H The relationship between EIF4A3 and circ_0086296 was evaluated via Pearson’s correlation analysis (n = 9). I, J The colocalization of circ_0086296 and EIF4A3 in plaque tissues (I) (scale bar, 50 μm); and in HUVECs (J) was verified via fluorescence in situ hybridization (FISH) (scale bar, 10 μm). **p < 0.001, ^##p < 0.001 versus the relative control group