Figure 4.

Silencing PoLaeA1 decreased the intracellular ROS levels. (A) DCFH-DA staining (scale bar = 100 μm). The tested strains cultured on CYM plates were stained with the fluorescent H₂O₂ probe DCFH-DA. (B) The H₂O₂ fluorescence values. The fluorescence was analyzed with ZEN 3.3 (blue edition). (C) Intracellular H₂O₂ levels. The tested strains were cultured in liquid CYM at 28 °C for 5 days. The grounded mycelia were used to determine the intracellular H₂O₂ levels with a commercial hydrogen peroxide assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). The values are the mean ± SD (n = 3). Different lowercase letters indicate significant differences between the strains (p < 0.05, according to Dunnett’s multiple comparisons test).