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. 2022 Aug 25;10(9):1394. doi: 10.3390/vaccines10091394

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Results of qmosRT-PCR assay using plasmid refence standards for quantification of nOPV2-c1_295 mutants, generated from the virus-spiked samples: (A) the mutant percentages generated by qmosRT-PCR are plotted against the expected percentages of mutants and (B) the mutant percentages generated by qmosRT-PCR are plotted against the results of Illumina sequencing (NGS). The correlation line is drawn for the averages of the qmosRT-PCR results. The results of different qmosRT-PCR runs (Table 4) are presented with different colors.