Table 3.
Quantification of nOPV2-c1_171 mutants in the virus-spiked samples by qmosRT-PCR assay using plasmids as reference standards.
| Expected Mutant % (GC #) | NGS (Set 1 and Set 2) | qmosRT-PCR Runs-Mutant % | ||||||
|---|---|---|---|---|---|---|---|---|
| Set 1 of Spiked Samples | Set 2 of Spiked Samples | Average ± SD | ||||||
| Mutant % ± SD | Run 1 | Run 2 | Run 3 | Run 4 | Run 5 | Run 6 | ||
| 75.12 | 76.69 ± 0.27 | 89.68 | 81.88 | 80.04 | 89.98 | 89.42 | 89.96 | 86.83 ± 4.59 |
| 50.34 | 52.63 ± 2.25 | 68.42 | 53.02 | 54.33 | 69.74 | 68.30 | 69.08 | 63.81 ± 7.88 |
| 30.41 | 28.37 ± 0.59 | 41.17 | 31.03 | 32.49 | 49.39 | 47.39 | 49.81 | 41.88 ± 8.44 |
| 17.00 | 16.51 ± 1.08 | 22.07 | 13.31 | 14.46 | 28.88 | 27.00 | 32.27 | 23.00 ± 7.80 |
| 9.03 | 8.28 ± 0.02 | 10.25 | 6.94 | 7.63 | 14.37 | 13.09 | 15.99 | 11.38 ± 3.69 |
| 4.67 | 3.94 ± 0.26 | 2.56 | 2.36 | 2.27 | 5.47 | 4.86 | 7.49 | 4.17 ± 2.13 |
| 2.37 | 2.22 ± 0.35 | 0.28 | 0.55 | 0.42 | 1.24 | 1.07 | 1.68 | 0.87 ± 0.54 |
| 1.20 | 0.63 ± 0.21 | 0.01 | 0.12 | 0.07 | 0.16 | 0.13 | 0.34 | 0.14 ± 0.11 |
| 0.60 | 0.66 ± 0.29 | ND | 0.005 | 0.01 | 0.02 | 0.01 | 0.02 | 0.01 ± 0.01 |
| 0.30 | 0.42 ± 0.10 | ND | ND | ND | ND | ND | ND | ND |
| 0.15 | ND | ND | ND | ND | ND | ND | ND | ND |
Note: GC #, genome copy number; NGS, next-generation sequencing (Illumina sequencing); SD, standard deviation; ND, not detected.