Table 6.
Quantification of nOPV2 mutants in nOPV2 batches by qmosRT-PCR assay using plasmids as reference standards.
| nOPV2 Lots | nOPV2_295 Mutants, % ± SD | nOPV2_143 Mutants, % ± SD | nOPV2_171 Mutants, % ± SD | ||||||
|---|---|---|---|---|---|---|---|---|---|
| NGS | qmosRT-PCR | R2 | NGS | qmosRT-PCR | R2 | NGS | qmosRT-PCR | R2 | |
| nPOL2056C-c2 | 14.82 ± 0.89 | 27.49 ± 0.35 | 0.97 | ND | ND | 0.85 | ND | ND | 0.99 |
| nPOL2018C | 14.61 ± 0.52 | 23.98 ± 0.17 | 1.67 ± 0.08 | 1.44 ± 0.68 | 9.31 ± 0.27 | 7.71 ± 0.05 | |||
| nPOL2038C-c2 | 2.07 ± 0.13 | 0.08 ± 0.03 | ND | ND | ND | 0.02 ± 0.00 | |||
| nPOL2016C | 45.83 ± 1.17 | 62.49 ± 2.16 | 0.21 ± 0.08 | ND | 0.9 ± 0.08 | 0.14 ± 0.08 | |||
| nPOL2B0419 | 32.71 ± 0.40 | 55.44 ± 1.02 | 1.19 ± 0.04 | 0.34 ± 0.16 | 24.18 ± 0.30 | 25.72 ± 0.72 | |||
| nPOL2B0519 | 37.92 ± 0.60 | 60.36 ± 1.24 | 1.11 ± 0.10 | 0.50 ± 0.24 | 28.83 ± 0.65 | 29.17 ± 3.48 | |||
| nPOL2-119C | 4.97 ± 0.24 | 4.21 ± 0.69 | 0.99 ± 0.14 | 0.42 ± 0.37 | 3.7 ± 0.31 | 1.49 ± 0.36 | |||
| nPOL2-219C | 7.37 ± 0.48 | 7.86 ± 0.77 | 1.93 ± 0.29 | 1.60 ± 0.30 | 5.26 ± 0.34 | 2.30 ± 0.79 | |||
| nPOL2-319C | 7.35 ± 0.40 | 7.89 ± 0.57 | 1.63 ± 0.15 | 1.14 ± 0.52 | 5.23 ± 0.47 | 2.25 ± 0.65 | |||
Note: NGS, next-generation sequencing (Illumina sequencing); SD, standard deviation; R2, coefficient of determination; ND, not detected.