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. 2022 Sep 22;10:152. doi: 10.1186/s40168-022-01344-6

Fig. 2.

Fig. 2

Maximum likelihood phylogeny of the plyNCR marker among strains of the Streptococcus mitis group. The ML tree was inferred using the generalized time reversible (GTR) + gamma model with FastTree and is unrooted with unscaled branches. A zoomed-in subtree (dashed rectangle) shows a cladistic clustering and a portion of the sequence alignment from five pneumococci (in blue) and 18 viridans group streptococci (VGS; S. mitis and S. pseudopneumoniae in red and yellow, respectively) with the latter having characteristic deletions. Above the subtree, the plyNCR is illustrated as a non-coding region flanked by partial sections of the conserved genes, yeeN (hypothetical regulatory protein) and ply (pneumolysin)