FIG. 1.

Outer membrane proteins from the Gifsy-resistant mutants and the ompC⁺ plasmid-complemented strains. The region of the gel corresponding to 30- to 40-kDa proteins is shown. All Salmonella strains were galE496 Gifsy-1- and Gifsy-2-cured derivatives of serovar Typhimurium strain 14028 (8). Plasmid-bearing strains also contained the zjg-8103::pir⁺allele (9). Lanes: A, E. coli (DH5αλpir⁺); B, pompC⁺ in E. coli (“p” before the gene indicates that the gene was carried on a plasmid); C, ompC⁺; D, pompC⁺ in ompC⁺; E, ompC396::Tn10; F, pompC⁺ in ompC396::Tn10; G, Δ[(sspH2)*MudCm*(ompC)]; H, pompC⁺ in Δ[(sspH2)*MudCm*(ompC)].