Table 1.
Oligonucleotide sequences and PCR conditions for mycobacterial speciation of oronasal swab cultures from African buffaloes.
| Primer Name | Forward Primer Sequence (5′–3′) |
Reverse Primer Sequence (5′–3′) |
Annealing Temperature | Product Size |
|---|---|---|---|---|
| rpoB | GGCAAGGTCACCCCGAAGGG | AGCGGCTGCTGGGTGATCATC | 64 °C | ±764 bp |
| hsp65 | ACCAACGATGGTGTGTCCAT | CTTGTCGAACCGCATACCCT | 60 °C | ±439 bp |
| esat-6 | CATGACAGAGCAGCAGTG | GCCCTATGCGAACATCCC | 60 °C | ±292 bp |
| cfp-10 | GTAGCCCGGGATGGCAGAGATGAAGACCGATGCC | TCAGAAGCCCATTTGCGAGGACAGC | 60 °C | ±300 bp |
| bp; base pairs | ||||