Table 2.

Antibodies used for Protein Expression Analyses.

Protein Size (kDa)	IHC Dilution	Clone	Catalog No.	Supplier	Significance	Antibody
289	–	–	#2971	Cell Signaling	Active mTOR kinase	Anti-phospho(Ser2448)-mTOR
289	0.1111111	49F9	#2976	Cell Signaling	Active mTOR kinase	Anti-phospho(Ser2448)-mTOR
289	–	7C10	#2983	Cell Signaling	Amount of mTOR protein	Anti-mTOR
32	0.1111111	–	#2211	Cell Signaling	mTORC1 activity	Anti-phospho(Ser235/236)-S6
60	–	D9E	#4060	Cell Signaling	mTORC2 activity	Anti-phospho(Ser473)-Akt
60	–	C67E7	#4691	Cell Signaling	Amount of Akt protein	Anti-Akt (pan)
200	–	–	#2140	Cell Signaling	Amount of mTORC2	Anti-Rictor
200	0.7361111	1G3P2C9	A500-002	Bethyl	Amount of mTORC2	Anti-Rictor
55	0.3194444	–	ab652	Abcam	Glucose uptake	Anti-GLUT1
102	–	C35C4	#2024	Cell Signaling	Glycolysis	Anti-HXK1
102	0.1805556	C64G5	#2867	Cell Signaling	Glycolysis	Anti-HXK2
80	0.1111111	D4B2	#8164	Cell Signaling	Glycolysis	Anti-PFKP
37	–	C4B5	#3582	Cell Signaling	Warburg effect	Anti-LDHA
37	–	60H11	ab85319	Abcam	Reverse Warburg effect	Anti-LDHB
59	0.1111111	EPR6292	ab133525	Abcam	Pentose phosphate pathway	Anti-G6PD
55–60	0.1805556	EP7212	ab156876	Abcam	Glutaminolysis	Anti-GLS
78	0.1805556	D19C6	#3658	Cell Signaling	Acetate utilization	Anti-ACSS2
88	0.3888889	8F6AE9	ab128568	Abcam	β-oxidation of fatty acids	Anti-CPT1A
52	0.1111111	3D5	ab14730	Abcam	Oxidative phosphorylation	Anti-ATPB
17	1.4305556	3E11	#4850	Cell signaling	Terminal oxidation	Anti-COX IV
16	0.1111111	D8T4N	#42406	Cell Signaling	Mitochondrial marker	Anti-TOM20
45	–	AC-74	A2228	Sigma-Aldrich	Loading control	Anti-β-actin

Abbreviations: ACSS2, acetyl-coenzyme A synthetase short-chain family member 2; Akt, protein kinase B; ATPB, β-F1-ATPase; COX IV, cytochrome c oxidase complex IV; CPT1A, carnitine palmitoyltransferase 1A; G6PD, glucose-6-phosphate dehydrogenase; GLS, glutaminase; GLUT1, glucose transporter 1; HXK1, hexokinase 1; HXK2, hexokinase 2; IHC, immunohistochemistry; LDHA, lactate dehydrogenase A; LDHB, lactate dehydrogenase B; mTOR, mammalian target of rapamycin; PFKP, phosphofructokinase; Rictor, rapamycin-insensitive companion of mTOR; TOM20, translocase of the outer mitochondrial membrane complex subunit 20; WES, Wes^TM Simple analysis.