Figure 3.

TH1902 inhibits CSC migration. (A) Pictures of wells bearing confluent layers of hTNBCSC were scratched to denude one area of cells, rinsed with serum-free medium and then treated for 2 h with vehicle (DMSO), 2 µM docetaxel (Doce) or 1 μM TH1902 (equivalent in docetaxel content to the docetaxel treatment) in serum-free media. Cells were then rinsed and incubated in fresh complete media. At 0 and 24 h following the initial wound, cells were washed and then photographed using a phase contrast microscope at a 4× magnification. (B) Quantification was performed with ImageJ. Data were obtained from three different experiments and are represented as means +/− SEM (n = 3); statistical analysis was performed for each time point using t-test (*** signifies p < 0.001 for differences between TH1902 and other conditions).