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. 2022 Sep 23;22:236. doi: 10.1186/s12902-022-01155-8

Table 1.

RT-q PCR primers for each target gene

Gene Sequence (5′-3′) Tm
FXR

F: CGGCTGTCAGGATTTGTGC

R: GAAGCCCAGGTTGGAATAGTAAG

60 °C
TGR5

F: ACTCTGTTATCGCTCATCTCATTG

R: AAGCACTCGTAGACACCTTTGG

60 °C
VDR

F: CACCTGGCTGATCTTGTCAGT

R: CTGGTCATCAGAGGTGAGGTC

60 °C
GLP-1

F: CAAACCAAGATCACTGACAAGAAAT

R: GGGTTACACAATGCTAGAGGGA

60 °C
β-actin

F: GCACCACACCTTCTACAATGAGC

R: GGATAGCACAGCCTGGATAGCAAC

60 °C