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Quantitative 13C NMR spectrum of CMC used for preparation of CMCG (298 K, D2O). Resonance assignments are also indicated. The numbers 2s, 3s, and 6s represent the resonance lines at the positions substituted by the carboxymethyl groups. O-CM and N-CM refer to the carbonyl carbon resonances of the carboxymethyl groups substituted at the hydroxyl and amine groups of chitosan, respectively.
