Figure 3.

Characterization of the immune sera. Immune serum was used as the primary antibody in Western blots to detect recombinant murine PrP (a) or PrP in brain homogenates from terminally ill Rocky Mountains Laboratory (RML) mice (b). The immune serum recognizes recombinant PrP, as well as total PrP (PrP^C and PrP^Sc) and PrP^Sc in brain homogenates. (c) The immune serum also recognized PrP expressed on the surface of DT-40 cells stably transfected to express PrP. PrP-tryp: serum from a mouse immunized with PrP-VSG expressing trypanosomes; Control: serum from a mouse immunized with WT-VSG expressing trypanosomes; 6H4: monoclonal anti-PrP antibody. Red boxes indicate the di-, mono- and unglycosylated forms of PrP. Data from a representative PrP-tryp and a control serum (second bleed) are depicted in all three panels.