Figure 6.

Representative histology for transplants at late neurula stage with sibling control comparisons. Dorsal is facing up. The four regional marker genes XCG-1, Otx2, En-2, and Krox20 are expressed at the cement gland, eye and forebrain, midbrain, and rhombomeres 3 and 5 of the hindbrain, respectively. Purple stain marked with a solid arrowhead indicates gene expression colocalized with transplant tissue, purple stain marked with an empty arrowhead indicates endogenous gene expression, and blue stain indicates transplant incorporation. Late gastrula rotated embryos show decreased level and more restricted area of marker gene expression at correct locations compared with mid-gastrula sham and rotated embryos. Scale bar represents 250 µm.