Figure 10.

Histology scores for transplants at hatching stage. Histological sections were scored for expression levels of regional marker genes, both correct “New On” expression (A) and ectopic “Old Off” expression (B). Horizontal black lines represent the mean, and error bars represent the SEM. ** p < 0.01. N for each gene and condition is indicated along the x-axis in gray. Late gastrula rotated embryos have significantly less appropriate marker gene expression than mid-gastrula sham (p = 0.0064) and Rotated embryos (p = 0.0012). Late gastrula rotated embryos also have significantly more ectopic marker gene expression (p = 0.0022) than mid-gastrula rotated embryos.