Figure 13.

Histology scores for mid- and late gastrula rotated transplants at stage 14 to 16. Histological sections were scored for expression levels of regional marker genes, both correct “New On” expression (A) and ectopic “Old Off” expression (B). Horizontal black lines represent the mean, and error bars represent the SEM. * p < 0.05, *** p < 0.001, **** p < 0.0001. n = 3 for each stage and gene. For mid-gastrula rotated embryos, stage 15 embryos have significantly higher levels of correct marker gene expression than stage 14 embryos (p = 0.0100), and stage 16 embryos have significantly higher levels of expression than both stage 14 (p < 0.0001) and stage 15 (p = 0.0007) embryos. Stage 16 mid-gastrula rotated embryos also have significantly higher levels of correct marker gene expression than stage 16 late gastrula rotated embryos (p < 0.0001).