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. 2022 Sep 9;27(18):5848. doi: 10.3390/molecules27185848

Figure 7.

Figure 7

Morphology of neutrophils and neutrophil extracellular traps (NETs) in ex vivo blood after incubation (37 °C) with or without cationic antimicrobial peptides (CAMPs) in the absence or presence of neutrophil stimulation with PMA (100 nM). Concentration was 50 μM for pept_1, and 100 μM for pept_2, pept_3, pept_4 or pept_5. (A,C)—neutrophil (A) and NET (C) after 3 h blood incubation. Neutrophils (A) had similar morphological features, which are characteristic of resting cells, in control blood and blood supplemented with any of peptides. NETs (C) also did not differ in shape, size and structure in control blood and any of the peptide-exposed blood. (B,D)—neutrophil (B) and NET (D) after 3 h blood incubation, with 100 nM PMA added at the 1 h. Neutrophils (B) both in blood with and without peptides displayed similar morphological features characteristic of activated neutrophils (swollen shape, cytoplasmic vacuolization, and swollen nuclei). NETs (D) were also alike in control blood and blood supplemented with any of peptides. Shown are Romanowsky-stained smears of whole blood. Scale bar in each subfigure: 10 μm.