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. 2022 Sep 9;27(18):5848. doi: 10.3390/molecules27185848

Figure 8.

Figure 8

NETosis in ex vivo blood upon 3 h incubation (37 °C) with or without cationic antimicrobial peptides (CAMPs) in the absence (light and dark blue bars) or presence (light and dark ochre bars) of neutrophil stimulation with PMA (100 nM). Concentration was 50 μM for pept_1, and 100 μM for pept_2, pept_3, pept_4 or pept_5. Shown are representative data for blood from one or another healthy volunteer. In each group of bars, from left to right: blood; blood + peptide; blood + PMA; blood + peptide + PMA. No significant difference in NET concentration after 3 h incubation was revealed between control blood and blood supplemented with peptides (light and dark blue bars). NET formation in response to PMA was also similar in control blood and blood with peptides (light and dark ochre bars). The results obtained in triplicate are shown as mean ± SD.