. 2022 Sep 5;14(9):1872. doi: 10.3390/pharmaceutics14091872

Table 4.

Minimum inhibitory, biofilm-inhibitory and biofilm-eradication concentrations of EO–EO and EO–clotrimazole combinations against Candida species.

Species	Combination	MIC (µg/mL)		Biofilm (µg/mL)
Species	Combination	Isolated MIC *	Combined MIC **	Isolated MBIC *	Combined MBIC **	Isolated MBEC *	Combined MBEC **
C. albicans ATCC 90028	M. alternifolia	2000	250	2000	62.5	4000	62.5
C. albicans ATCC 90028	Clotrimazole	0.25	0.063	1	0.25	1	0.25
C. albicans SV 01	L. cubeba	2000	250	2000	125	4000	250
C. albicans SV 01	M. alternifolia	4000	1000	4000	2000	4000	1000
C. glabrata ATCC 2001	L. cubeba	2000	500	2000	2000	>4000	2000
C. glabrata ATCC 2001	C. limon	4000	1000	>4000	250	>4000	250
C. glabrata SV 02	L. cubeba	2000	250	>4000	1000	>4000	>1000
	M. alternifolia	4000	1000	4000	250	>4000	>250
	C. limon	4000	1000	>4000	4000	>4000	4000
	M. alternifolia	4000	1000	4000	250	>4000	250
C. krusei ATCC 6258	C. sempervirens	2000	1000	2000	4000	2000	>4000
	C. limon	4000	250	>4000	62.5	>4000	>250
	C. limon	1000	1000	>4000	4000	>4000	2000
	M. alternifolia	2000	1000	4000	250	>4000	500
C. parapsilosis SV 04	C. sempervirens	>4000	250	4000	500	>4000	125
C. parapsilosis SV 04	Clotrimazole	0.125	0.032	0.25	0.015	0.5	0.063

Isolated MBIC and MBEC (µg/mL): able to reduce by ≥90% optical density (OD) compared to control free of EO and clotrimazole. * Isolated: only one substance (EO or clotrimazole). ** Combined: MIC of the combination (EO–EO or EO–clotrimazole) that resulted in synergism.