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. 2022 Sep 4;14(9):1961. doi: 10.3390/v14091961

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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RNA levels of SP as detected by qPCR against a standard curve in whole blood and brain. Top: Whole blood. Bottom: Whole brain tissue homogenate. Error bars represent the standard deviations. Significant differences between WNV-infected and mock mice were detected within 3 DPI (p = 0.0092) and remained significant at 4 DPI (p = 0.0007), 5 DPI (p = 0.0009), and 6 DPI (0.0012), while SP copy numbers’ increases in the brain are not detectable until 6 DPI (p = 0.0031). N = 10 per group per day, and N = 18 WNV-infected mice are included in the analyses of D6 and D7 due to early attrition. *: p < 0.01.